WATCH: Jimmy Fallon, Stephen Colbert React to Orlando Shooting

Stephen Colbert: Despair Is A Victory For HateStephen opens The Late Show with his thoughts on the heartbreaking events in Orlando. "Subscribe To ""The Late Show"" Channel HERE: bit.ly/ColbertYouTube For more content from ""The Late Show with Stephen Colbert"", click HERE: bit.ly/1AKISnR Watch full episodes of ""The Late Show"" HERE: bit.ly/1Puei40 Download the Colbert App HERE: apple.co/1Qqgwk4 Like ""The Late Show"" on…2016-06-14T07:35:00.000Z

As they have had to do several times over the past year, Stephen Colbert and Jimmy Fallon had to talk to their viewers about a tragedy. The two comedians opened their shows with statements on the mass shooting in Orlando, the worst in U.S. history, at the gay nightclub Pulse. Forty-nine people were killed and 53 were injured.

Colbert had the columns of the Late Show set lit in the colors of the rainbow. In his monologue, he said that most of his viewers know what people will say after a tragedy like this.

“It’s as if there’s a national script that we have learned,” Colbert said. “And I think that by accepting the script, we tacitly accept that the script will end the same way every time, with nothing changing.” He continued that accepting this with despair “is a victory for hate,” which “wants us to be too weak to change anything.”

Fallon gave a tearful monologue to open The Tonight Show. As a new father, Fallon wondered what he could tell his kids about the tragedy and suggested there was a “lesson of tolerance” to take from this. “We need to get back to being brave enough to accept that we have different opinions and that’s OK because that’s what America is built on.”

Jimmy Fallon Addresses Orlando Nightclub ShootingJimmy shares his thoughts in the wake of the mass shooting at Pulse nightclub in Orlando, Florida. Subscribe NOW to The Tonight Show Starring Jimmy Fallon: bit.ly/1nwT1aN Watch The Tonight Show Starring Jimmy Fallon Weeknights 11:35/10:35c Get more Jimmy Fallon: Follow Jimmy: Twitter.com/JimmyFallon Like Jimmy: Facebook.com/JimmyFallon Get more The Tonight Show Starring Jimmy Fallon: Follow…2016-06-14T03:38:44.000Z

Seth Meyers began Late Night with a special “Closer Look” segment on the shooting.

VideoVideo related to watch: jimmy fallon, stephen colbert react to orlando shooting2016-06-14T06:46:59-04:00

On Sunday night, John Oliver was the first host to discuss the shooting.

John Oliver – Orlando ShootingJohn Oliver's Last Week Tonight on HBO2016-06-13T04:52:59.000Z